Rat CDH5(Cadherin 5) ELISA Kit

 

Rat CDH5(Cadherin 5) ELISA Kit

Size

96T

Catalog no.

ELK7048

Price

608 EUR

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Assay length

3h

Sensitivity

27pg/mL

Assay Type

Sandwich

Standard

5000pg/mL

Detection range

78-5000pg/mL

Latin name

Rattus norvegicus

Research Area

CD & Adhesion molecule;Tumor immunity;Infection immunity;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Alternative Names

CD144; 7B4; Cadherin 5 Type 2; VE-Cadherin; Cadherin,Vascular Endothelial

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin 5 (CDH5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cadherin 5 (CDH5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cadherin 5 (CDH5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cadherin 5 (CDH5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.