Cattle HSPA1B(Heat Shock 70kDa Protein 1B) ELISA Kit

3h

100ng/mL

Sandwich

0.53ng/mL

1.56-100ng/mL

Infection immunity;

HSP70-2; HSP-A1B; Heat Shock Protein 72

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 1B (HSPA1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 1B (HSPA1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70kDa Protein 1B (HSPA1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 70kDa Protein 1B (HSPA1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.