Cattle DEFb2(Defensin Beta 2) ELISA Kit

 

Cattle DEFb2(Defensin Beta 2) ELISA Kit

Size

96T

Catalog no.

ELK7064

Price

738 EUR

Buy at gentaur.com
Assay length

3h

Sensitivity

5.7pg/mL

Assay Type

Sandwich

Standard

1000pg/mL

Detection range

15.6-1000pg/mL

Research Area

Infection immunity;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Alternative Names

B-DF2; DEFB2; BD2; DEF-B2; HBD2; DEFB102; HBD-2; SAP1; DEFB4A; DEFB4; Skin-Antimicrobial Peptide 1

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Description

human, mouse or other beta β- and alpha α- defensins are expressed by neutrophils ( α- ), lymphocytes and epithelial cells. DEFA DEFB genes defend the host against pathogens. GENTAUR supplies anti-defensins, monclonals, polyclonals antisera and recombinant DEFs or peptides of defensin.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 2 (DEFβ2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 2 (DEFβ2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 2 (DEFβ2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Defensin Beta 2 (DEFβ2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.