3h
96T
ELK7125
608 EUR
3h
10ng/mL
Sandwich
0.053ng/mL
Hematology;
Mus musculus
0.156-10ng/mL
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
BAG6; Protein G3; Protein Scythe; BCL2-associated athanogene 6; BAG family molecular chaperone regulator 6; Large proline-rich protein BAG6
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HLA-B Associated Transcript 3 (BAT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to HLA-B Associated Transcript 3 (BAT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HLA-B Associated Transcript 3 (BAT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HLA-B Associated Transcript 3 (BAT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.