2h
96T
ELK7129
738 EUR
2h
1000pg/mL
4.35pg/mL
12.35-1000pg/mL
Competitive Inhibition
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Metabolic pathway;Endocrinology;Gastroenterology;Hormone metabolism;
Pigs and the smaller guinea pigs are frequent used as models for humans.
GAST; GAS; Gastrin component I; Big gastrin; Gastrin component II; Gastrin component III
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Guinea pig ELISA kits for plasma and sera samples are used to study human genes through the guinea pig model (Cavia porcellus), also called the cavy rodent model. After mouzes and rats Guinea pigs are easy in maintained laboratory animals. cDNAs of Guinea pigs are also very popular.
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gastrin (GT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Gastrin (GT) and unlabeled Gastrin (GT) (Standards or samples) with the pre-coated antibody specific to Gastrin (GT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Gastrin (GT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gastrin (GT) in the sample.