Rat DSTYK(Dual Serine/Threonine And Tyrosine Protein Kinase) ELISA Kit

 

Rat DSTYK(Dual Serine/Threonine And Tyrosine Protein Kinase) ELISA Kit

Size

96T

Catalog no.

ELK7203

Price

608 EUR

Buy at gentaur.com
Assay length

3h

Standard

20ng/mL

Assay Type

Sandwich

Sensitivity

0.113ng/mL

Detection range

0.312-20ng/mL

Research Area

Enzyme & Kinase;

Latin name

Rattus norvegicus

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Alternative Names

RIPK5; Dusty PK; RIP5; SgK496; Receptor Interacting Protein Kinase 5; RIP-homologous kinase; Sugen kinase 496; Receptor-interacting serine/threonine-protein kinase 5

Test

The duality of this product lies in it's dual specificity to the gene, pH or protein. Mainly in phosphorylation by specific phosphatases.ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Description

Serine protease, D- or L-serine arginine rich enzyme of serine threonine kinase with serine that is encoded by the codons UCU, UCC, UCA, UCG, AGU and AGC is an ɑ-amino acid that is used in the biosynthesis of proteins. It contains an α-amino group (which is in the protonated −NH+ 3 form under biological conditions), a carboxyl group. It is non-essential in humans, meaning the body can synthesize it.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.