3h
Cattle INHbE(Inhibin Beta E) ELISA Kit
Cattle INHbE(Inhibin Beta E) ELISA Kit
Assay length
Sensitivity
5.3pg/mL
Assay Type
Sandwich
Standard
1000pg/mL
Research Area
Endocrinology;
Detection range
15.62-1000pg/mL
Alternative Names
INH-bE; Activin beta-E chain
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inhibin Beta E (INHbE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inhibin Beta E (INHbE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inhibin Beta E (INHbE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inhibin Beta E (INHbE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.