2h
Rat NMU(Neuromedin U) ELISA Kit
Rat NMU(Neuromedin U) ELISA Kit
Assay length
Alternative Names
NM-U
Sensitivity
48.2pg/mL
Standard
10000pg/mL
Detection range
123.5-10000pg/mL
Latin name
Rattus norvegicus
Assay Type
Competitive Inhibition
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Research Area
Endocrinology;Cardiovascular biology;Neuro science;Gastroenterology;Hormone metabolism;Bone metabolism;
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neuromedin U (NMU) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neuromedin U (NMU) and unlabeled Neuromedin U (NMU) (Standards or samples) with the pre-coated antibody specific to Neuromedin U (NMU). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neuromedin U (NMU) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Neuromedin U (NMU) in the sample.