Rabbit ATP1a1(ATPase, Na+/K+ Transporting Alpha 1 Polypeptide) ELISA Kit

 

Rabbit ATP1a1(ATPase, Na+/K+ Transporting Alpha 1 Polypeptide) ELISA Kit

Size

96T

Catalog no.

ELK7384

Price

738 EUR

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Assay length

3h

Standard

20ng/mL

Assay Type

Sandwich

Sensitivity

0.115ng/mL

Detection range

0.312-20ng/mL

Research Area

Signal transduction;

Latin name

Oryctolagus cuniculus

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Alternative Names

Sodium pump subunit alpha-1; Na(+)/K(+) ATPase alpha-1 subunit; Sodium/potassium-transporting ATPase subunit alpha-1

Description

The Rabbit ATP1a1(ATPase, Na+/K+ Transporting Alpha 1 Polypeptide) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

About

Rabbits are used for polyclonal antibody production by ELK Biotech. Rabbit antibodies are very stable and can be stored for several days at room temperature. ELK Biotech adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1α1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1α1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1α1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1α1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.