Pig sIgA(Secretory Immunoglobulin A) ELISA Kit

2h

S-IgA

100ng/mL

0.53ng/mL

1.23-100ng/mL

Competitive Inhibition

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Pigs and the smaller guinea pigs are frequent used as models for humans.

Infection immunity;Immune molecule;Reproductive science;Genetic science;Dermatology;Immunodeficiency;

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Secretory Immunoglobulin A (sIgA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Secretory Immunoglobulin A (sIgA) and unlabeled Secretory Immunoglobulin A (sIgA) (Standards or samples) with the pre-coated antibody specific to Secretory Immunoglobulin A (sIgA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Secretory Immunoglobulin A (sIgA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Secretory Immunoglobulin A (sIgA) in the sample.