Pig EDN1(Endothelin 1) ELISA Kit

 

Pig EDN1(Endothelin 1) ELISA Kit

Size

96T

Catalog no.

ELK7427

Price

738 EUR

Buy at gentaur.com
Assay length

2h

Standard

1000pg/mL

Sensitivity

4.74pg/mL

Detection range

12.35-1000pg/mL

Assay Type

Competitive Inhibition

Research Area

Endocrinology;Cardiovascular biology;

Alternative Names

ET1; PPET1; Preproendothelin-1; Big endothelin-1

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

About

Pigs and the smaller guinea pigs are frequent used as models for humans.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Endothelin 1 (EDN1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Endothelin 1 (EDN1) and unlabeled Endothelin 1 (EDN1) (Standards or samples) with the pre-coated antibody specific to Endothelin 1 (EDN1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Endothelin 1 (EDN1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Endothelin 1 (EDN1) in the sample.