3h
Human EXOSC7(Exosome Component 7) ELISA Kit
Human EXOSC7(Exosome Component 7) ELISA Kit
Assay length
Tissue
exosome
Standard
10ng/mL
Assay Type
Sandwich
Sensitivity
0.065ng/mL
Detection range
0.156-10ng/mL
Research Area
Signal transduction;
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Alternative Names
Rrp42p; RRP42; EAP1; p8; hRrp42p; Ribosomal RNA-processing protein 42; Exosome complex component RRP42
Gene
Exosomes are 50 nm small lipoprotein vesicles containing lots of microRNAs. The diagnostic value of exosomic miRNAs is due to their role in signal transduction and cell differentiation. Exoslomal total RNA can be analyzed by next gen sequencing to perform a diagnostic micro RNA profile.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Exosome Component 7 (EXOSC7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Exosome Component 7 (EXOSC7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Exosome Component 7 (EXOSC7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Exosome Component 7 (EXOSC7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.