Cattle C3(Complement Component 3) ELISA Kit

3h

200ng/Ml

Sandwich

1.28ng/mL

3.12-200ng/Ml

Signal transduction;Infection immunity;Immune molecule;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ASP; CPAMD1; Complement Protein C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Acylation stimulating protein

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Complement Component 3 (C3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Complement Component 3 (C3) and unlabeled Complement Component 3 (C3) (Standards or samples) with the pre-coated antibody specific to Complement Component 3 (C3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Complement Component 3 (C3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Complement Component 3 (C3) in the sample.