Cattle ACTH(Adrenocorticotropic Hormone) ELISA Kit

2h

1000pg/mL

4.57pg/mL

12.35-1000pg/mL

Competitive Inhibition

Corticotropin; Adrenocorticotropin

Endocrinology;Hormone metabolism;Urology;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Adrenocorticotropic hormone (ACTH), or corticotrophins.Hormone releasing factors and releasing hormones are  signaling molecules produced by glands in multicellular organisms. The glands that secrete Luteinizing hormones LHRG and LH, FSH comprise the endocrine signaling system. The term growth hormone releasing hormone GHRH is sometimes extended to include chemicals produced by cells that affect the same cell (autocrine or intracrine signaling) or nearby cells (paracrine signaling). Human recombinant LHRG and GHRH are produced in E. coli or in yeast cells.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Adrenocorticotropic Hormone (ACTH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Adrenocorticotropic Hormone (ACTH) and unlabeled Adrenocorticotropic Hormone (ACTH) (Standards or samples) with the pre-coated antibody specific to Adrenocorticotropic Hormone (ACTH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adrenocorticotropic Hormone (ACTH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Adrenocorticotropic Hormone (ACTH) in the sample.