Pig MIP3a(Macrophage Inflammatory Protein 3 Alpha) ELISA Kit

3h

500pg/mL

2.9pg/mL

Sandwich

7.8-500pg/mL

Cytokine;Infection immunity;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Pigs and the smaller guinea pigs are frequent used as models for humans.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

The MIP3a(Macrophage Inflammatory Protein 3 Alpha) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

MIP3 Alpha， CCL20; CKb4; LARC; MIP3-A; SCYA20; ST38; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 20; Beta-Chemokine Exodus-1; Liver And Activation-Regulated Chemokine

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 3 Alpha (MIP3α). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 3 Alpha (MIP3α). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 3 Alpha (MIP3α), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Inflammatory Protein 3 Alpha (MIP3α) in the samples is then determined by comparing the O.D. of the samples to the standard curve.