2h
Pig INHA(Inhibin A) ELISA Kit
Pig INHA(Inhibin A) ELISA Kit
Assay length
Alternative Names
INH-A
Standard
1000pg/mL
Sensitivity
5.16pg/mL
Detection range
12.35-1000pg/mL
Assay Type
Competitive Inhibition
Research Area
Endocrinology;Reproductive science;Hormone metabolism;
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About
Pigs and the smaller guinea pigs are frequent used as models for humans.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Inhibin A (INHA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Inhibin A (INHA) and unlabeled Inhibin A (INHA) (Standards or samples) with the pre-coated antibody specific to Inhibin A (INHA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Inhibin A (INHA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Inhibin A (INHA) in the sample.