3h
96T
ELK7732
608 EUR
3h
10ng/mL
Sandwich
0.056ng/mL
0.156-10ng/mL
Rattus norvegicus
CD & Adhesion molecule;
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
The ITGa4(Integrin Alpha 4) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
CD49d; CD49-D; ITG-A4; IA4; Alpha 4 Subunit Of VLA-4 Receptor; CD49 antigen-like family member D; Integrin alpha-IV
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Alpha 4 (ITGα4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Alpha 4 (ITGα4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Alpha 4 (ITGα4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Alpha 4 (ITGα4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.