Gallus CD4(Cluster Of Differentiation 4) ELISA Kit

3h

10ng/mL

Sandwich

0.061ng/mL

0.156-10ng/mL

Signal transduction;CD & Adhesion molecule;Immune molecule;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

CD4mut; p55; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 4 (CD4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 4 (CD4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 4 (CD4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cluster Of Differentiation 4 (CD4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.