Gallus CD3d(Cluster Of Differentiation 3d) ELISA Kit

3h

10ng/mL

Sandwich

0.055ng/mL

0.156-10ng/mL

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

CD3-DELTA; T3D; T-Dell Surface Glycoprotein CD3 Delta Chain; T-cell receptor T3 delta chain

Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 3d (CD3d). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 3d (CD3d). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 3d (CD3d), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cluster Of Differentiation 3d (CD3d) in the samples is then determined by comparing the O.D. of the samples to the standard curve.