Rat HPEPT1(Hydrogen Ion/Peptide Transporter 1) ELISA Kit

3h

10ng/mL

Sandwich

0.056ng/mL

0.156-10ng/mL

Rattus norvegicus

Signal transduction;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

SLC15A1; HPECT1; PEPT1; Solute Carrier Family 15 Member 1,Oligopeptide Transporter; Oligopeptide transporter, small intestine isoform; Intestinal H(+)/peptide cotransporter

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hydrogen Ion/Peptide Transporter 1 (HPEPT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hydrogen Ion/Peptide Transporter 1 (HPEPT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hydrogen Ion/Peptide Transporter 1 (HPEPT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hydrogen Ion/Peptide Transporter 1 (HPEPT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.