General 5-HT(5-Hydroxytryptamine) ELISA Kit

 

General 5-HT(5-Hydroxytryptamine) ELISA Kit

Size

96T

Catalog no.

ELK7830

Price

526 EUR

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Assay length

2h

Standard

900ng/mL

Sensitivity

3.97ng/mL

Detection range

11.11-900ng/mL

Assay Type

Competitive Inhibition

Alternative Names

5HT; Serotonin; N-Methyl-Gamma

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Research Area

Signal transduction;Infection immunity;Neuro science;Gastroenterology;

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 5-Hydroxytryptamine (5-HT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 5-Hydroxytryptamine (5-HT) and unlabeled 5-Hydroxytryptamine (5-HT) (Standards or samples) with the pre-coated antibody specific to 5-Hydroxytryptamine (5-HT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 5-Hydroxytryptamine (5-HT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 5-Hydroxytryptamine (5-HT) in the sample.