General FA(Folic Acid) ELISA Kit

 

General FA(Folic Acid) ELISA Kit

Size

96T

Catalog no.

ELK7834

Price

526 EUR

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Assay length

2h

Sensitivity

14.7pg/mL

Standard

10000pg/mL

Detection range

39.1-10000pg/mL

Assay Type

Competitive Inhibition

Research Area

Hematology;Reproductive science;Nutrition metabolism;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Alternative Names

VB9; Vitamin B9; Folacin; Folate; V; Itamin M; Vitamin M; Vitamin Bc; Pteroyl-L-Glutamic Acid; Pteroyl-L-Glutamate

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Folic Acid (FA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Folic Acid (FA) and unlabeled Folic Acid (FA) (Standards or samples) with the pre-coated antibody specific to Folic Acid (FA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Folic Acid (FA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Folic Acid (FA) in the sample.