2h
General Hyp(Hydroxyproline) ELISA Kit
General Hyp(Hydroxyproline) ELISA Kit
Assay length
Standard
5000ng/mL
Sensitivity
26.3ng/mL
Detection range
61.7-5000ng/mL
Assay Type
Competitive Inhibition
Research Area
Metabolic pathway;Nutrition metabolism;
Alternative Names
(2S,4R)-4-Hydroxyproline; L-Hydroxyproline(C5H9O3N)
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hydroxyproline (Hyp) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hydroxyproline (Hyp) and unlabeled Hydroxyproline (Hyp) (Standards or samples) with the pre-coated antibody specific to Hydroxyproline (Hyp). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hydroxyproline (Hyp) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Hydroxyproline (Hyp) in the sample.