2h
General E1(Estrone) ELISA Kit
General E1(Estrone) ELISA Kit
Assay length
Alternative Names
Oestrone
Standard
2000pg/mL
Sensitivity
8.86pg/mL
Detection range
24.69-2000pg/mL
Assay Type
Competitive Inhibition
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Research Area
Metabolic pathway;Endocrinology;Reproductive science;Genetic science;Hormone metabolism;
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Estrone (E1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Estrone (E1) and unlabeled Estrone (E1) (Standards or samples) with the pre-coated antibody specific to Estrone (E1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Estrone (E1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Estrone (E1) in the sample.