General 5-HIAA(5-Hydroxyindoleacetic Acid) ELISA Kit

 

General 5-HIAA(5-Hydroxyindoleacetic Acid) ELISA Kit

Size

96T

Catalog no.

ELK7838

Price

526 EUR

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Assay length

2h

Alternative Names

5HIAA

Standard

400ng/mL

Sensitivity

1.93ng/mL

Detection range

4.94-400ng/mL

Assay Type

Competitive Inhibition

Research Area

Metabolic pathway;Tumor immunity;Endocrinology;Urology;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 5-Hydroxyindoleacetic Acid (5-HIAA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 5-Hydroxyindoleacetic Acid (5-HIAA) and unlabeled 5-Hydroxyindoleacetic Acid (5-HIAA) (Standards or samples) with the pre-coated antibody specific to 5-Hydroxyindoleacetic Acid (5-HIAA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 5-Hydroxyindoleacetic Acid (5-HIAA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 5-Hydroxyindoleacetic Acid (5-HIAA) in the sample.