General 1,5-AG(1,5-Anhydroglucitol) ELISA Kit

2h

150ug/mL

0.6ug/mL

1.9-150ug/mL

Competitive Inhibition

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

LycoMark; 1-Deoxy-D-glucose; 1-Deoxy-D-glucopyranose; 1,5-Anhydro-D-glucitol; 1,5-Anhydrosorbitol; Aceritol; Polygalytol

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 1,5-Anhydroglucitol (1,5-AG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 1,5-Anhydroglucitol (1,5-AG) and unlabeled 1,5-Anhydroglucitol (1,5-AG) (Standards or samples) with the pre-coated antibody specific to 1,5-Anhydroglucitol (1,5-AG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 1,5-Anhydroglucitol (1,5-AG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 1,5-Anhydroglucitol (1,5-AG) in the sample.