2h
General LXA4(Lipoxin A4) ELISA Kit
General LXA4(Lipoxin A4) ELISA Kit
Assay length
Alternative Names
LX-A4
Standard
40000pg/mL
Sensitivity
175.4pg/mL
Detection range
493.8-40000pg/mL
Assay Type
Competitive Inhibition
Research Area
Signal transduction;Metabolic pathway;Infection immunity;
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Lipoxin A4 (LXA4) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Lipoxin A4 (LXA4) and unlabeled Lipoxin A4 (LXA4) (Standards or samples) with the pre-coated antibody specific to Lipoxin A4 (LXA4). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Lipoxin A4 (LXA4) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Lipoxin A4 (LXA4) in the sample.