General LXA4(Lipoxin A4) ELISA Kit

 

General LXA4(Lipoxin A4) ELISA Kit

Size

96T

Catalog no.

ELK7851

Price

526 EUR

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Assay length

2h

Alternative Names

LX-A4

Standard

40000pg/mL

Sensitivity

175.4pg/mL

Detection range

493.8-40000pg/mL

Assay Type

Competitive Inhibition

Research Area

Signal transduction;Metabolic pathway;Infection immunity;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Lipoxin A4 (LXA4) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Lipoxin A4 (LXA4) and unlabeled Lipoxin A4 (LXA4) (Standards or samples) with the pre-coated antibody specific to Lipoxin A4 (LXA4). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Lipoxin A4 (LXA4) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Lipoxin A4 (LXA4) in the sample.