2h
General MT(Melatonin) ELISA Kit
General MT(Melatonin) ELISA Kit
Assay length
Standard
1000pg/mL
Sensitivity
4.94pg/mL
Detection range
12.35-1000pg/mL
Assay Type
Competitive Inhibition
Alternative Names
N-Acetyl-5-Methoxytryptamine
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Research Area
Metabolic pathway;Tumor immunity;Neuro science;Hormone metabolism;
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Melatonin (MT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Melatonin (MT) and unlabeled Melatonin (MT) (Standards or samples) with the pre-coated antibody specific to Melatonin (MT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Melatonin (MT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Melatonin (MT) in the sample.