General TCPa(Alpha-Tocopherol) ELISA Kit

2h

100ug/mL

0.46ug/mL

1.23-100ug/mL

Competitive Inhibition

VE; Vitamin E; α-TCP; α-Tocopherol

Metabolic pathway;Nutrition metabolism;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

The General TCPa(Alpha-Tocopherol) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Alpha-Tocopherol (TCPα) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Alpha-Tocopherol (TCPα) and unlabeled Alpha-Tocopherol (TCPα) (Standards or samples) with the pre-coated antibody specific to Alpha-Tocopherol (TCPα). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Alpha-Tocopherol (TCPα) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Alpha-Tocopherol (TCPα) in the sample.