General CNCbl(Cyanocobalamin) ELISA Kit

2h

49.2pg/mL

10000pg/mL

123.5-10000pg/mL

Competitive Inhibition

Metabolic pathway;Nutrition metabolism;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

VB12; Vitamin B12; Cobalamin; α-5,6-Dimethylbenzimidazolyl Cobamidcyanide

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cyanocobalamin (CNCbl) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cyanocobalamin (CNCbl) and unlabeled Cyanocobalamin (CNCbl) (Standards or samples) with the pre-coated antibody specific to Cyanocobalamin (CNCbl). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cyanocobalamin (CNCbl) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cyanocobalamin (CNCbl) in the sample.