General 17-OHP(17-Hydroxyprogesterone) ELISA Kit

 

General 17-OHP(17-Hydroxyprogesterone) ELISA Kit

Size

96T

Catalog no.

ELK7875

Price

526 EUR

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Assay length

2h

Sensitivity

87.3pg/mL

Standard

20000pg/mL

Detection range

246.9-20000pg/mL

Assay Type

Competitive Inhibition

Research Area

Endocrinology;Reproductive science;Hormone metabolism;

Alternative Names

17OHP; 17-OH Progesterone; 17-Alpha-Hydroxyprogesterone

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 17-Hydroxyprogesterone (17-OHP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 17-Hydroxyprogesterone (17-OHP) and unlabeled 17-Hydroxyprogesterone (17-OHP) (Standards or samples) with the pre-coated antibody specific to 17-Hydroxyprogesterone (17-OHP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 17-Hydroxyprogesterone (17-OHP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 17-Hydroxyprogesterone (17-OHP) in the sample.