General ASD(Androstenedione) ELISA Kit

 

General ASD(Androstenedione) ELISA Kit

Size

96T

Catalog no.

ELK7877

Price

526 EUR

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Assay length

2h

Sensitivity

48.8pg/mL

Standard

10000pg/mL

Detection range

123.5-10000pg/mL

Assay Type

Competitive Inhibition

Alternative Names

4-Androstenedione; 4-Androstene-3,17-Dione

Research Area

Endocrinology;Reproductive science;Hormone metabolism;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Androstenedione (ASD) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Androstenedione (ASD) and unlabeled Androstenedione (ASD) (Standards or samples) with the pre-coated antibody specific to Androstenedione (ASD). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Androstenedione (ASD) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Androstenedione (ASD) in the sample.