2h
General NE(Noradrenaline) ELISA Kit
General NE(Noradrenaline) ELISA Kit
Assay length
Standard
5000pg/Ml
Sensitivity
26.1pg/mL
Detection range
61.7-5000pg/Ml
Assay Type
Competitive Inhibition
Alternative Names
NA; NAd; Norepinephrine; Norepi
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Research Area
Signal transduction;Infection immunity;Neuro science;Hormone metabolism;
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Noradrenaline (NE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Noradrenaline (NE) and unlabeled Noradrenaline (NE) (Standards or samples) with the pre-coated antibody specific to Noradrenaline (NE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Noradrenaline (NE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Noradrenaline (NE) in the sample.