2h
General LTB4(Leukotriene B4) ELISA Kit
General LTB4(Leukotriene B4) ELISA Kit
Assay length
Alternative Names
LT-B4
Sensitivity
89.3pg/mL
Standard
20000pg/mL
Detection range
246.9-20000pg/mL
Assay Type
Competitive Inhibition
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Research Area
Metabolic pathway;Infection immunity;Endocrinology;Hormone metabolism;
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Leukotriene B4 (LTB4) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Leukotriene B4 (LTB4) and unlabeled Leukotriene B4 (LTB4) (Standards or samples) with the pre-coated antibody specific to Leukotriene B4 (LTB4). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Leukotriene B4 (LTB4) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Leukotriene B4 (LTB4) in the sample.