2h
General VB6(Vitamin B6) ELISA Kit
General VB6(Vitamin B6) ELISA Kit
Assay length
Standard
300ng/mL
Sensitivity
1.42ng/mL
Alternative Names
Pyridoxine
Detection range
3.70-300ng/mL
Assay Type
Competitive Inhibition
Research Area
Nutrition metabolism;Hormone metabolism;
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Vitamin B6 (VB6) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Vitamin B6 (VB6) and unlabeled Vitamin B6 (VB6) (Standards or samples) with the pre-coated antibody specific to Vitamin B6 (VB6). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Vitamin B6 (VB6) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Vitamin B6 (VB6) in the sample.