General HVD3(25-Hydroxyvitamin D3) ELISA Kit

 

General HVD3(25-Hydroxyvitamin D3) ELISA Kit

Size

96T

Catalog no.

ELK7890

Price

526 EUR

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Assay length

2h

Standard

200ng/mL

Sensitivity

0.91ng/mL

Detection range

2.47-200ng/mL

Assay Type

Competitive Inhibition

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Research Area

Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;

Alternative Names

25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25(OH)D

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 25-Hydroxyvitamin D3 (HVD3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 25-Hydroxyvitamin D3 (HVD3) and unlabeled 25-Hydroxyvitamin D3 (HVD3) (Standards or samples) with the pre-coated antibody specific to 25-Hydroxyvitamin D3 (HVD3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 25-Hydroxyvitamin D3 (HVD3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 25-Hydroxyvitamin D3 (HVD3) in the sample.