3h
Cattle aLA(Alpha-Lactalbumin) ELISA Kit
Cattle aLA(Alpha-Lactalbumin) ELISA Kit
Assay length
Standard
40ng/mL
Assay Type
Sandwich
Sensitivity
0.285ng/mL
Detection range
0.625-40ng/mL
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Alternative Names
LALBA; LYZL7; Lactose Synthase B Protein; Lysozyme Like Protein 7
Research Area
Tumor immunity;Infection immunity;Endocrinology;Reproductive science;Hormone metabolism;
Description
The Cattle aLA(Alpha-Lactalbumin) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Lactalbumin (αLA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Lactalbumin (αLA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Lactalbumin (αLA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-Lactalbumin (αLA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.