General CML(Carboxymethyl Lysine) ELISA Kit

2h

5000ng/mL

25.9ng/mL

61.7-5000ng/mL

Competitive Inhibition

N(6)-Carboxymethyllysine

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Metabolic pathway;Endocrinology;Cardiovascular biology;Genetic science;Nutrition metabolism;

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Carboxymethyl Lysine (CML) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Carboxymethyl Lysine (CML) and unlabeled Carboxymethyl Lysine (CML) (Standards or samples) with the pre-coated antibody specific to Carboxymethyl Lysine (CML). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Carboxymethyl Lysine (CML) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Carboxymethyl Lysine (CML) in the sample.