Simian TIMP3(Tissue Inhibitors Of Metalloproteinase 3) ELISA Kit

6

3h

tissue

20ng/mL

Sandwich

0.112ng/mL

0.312-20ng/mL

Tumor immunity;Cardiovascular biology;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

SFD; TIMP Metallopeptidase Inhibitor 3; Metallopeptidase Inhibitor 3; Sorsby Fundus Dystrophy; Pseudoinflammatory

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tissue Inhibitors Of Metalloproteinase 3 (TIMP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tissue Inhibitors Of Metalloproteinase 3 (TIMP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tissue Inhibitors Of Metalloproteinase 3 (TIMP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.