3h
96T
ELK7917
738 EUR
3h
50ng/mL
Sandwich
0.32ng/mL
0.781-50ng/mL
MAC; Membrane Attack Complex
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Signal transduction;Metabolic pathway;Apoptosis;Infection immunity;
Pigs and the smaller guinea pigs are frequent used as models for humans.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Terminal Complement Complex C5b-9 (C5b-9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Terminal Complement Complex C5b-9 (C5b-9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Terminal Complement Complex C5b-9 (C5b-9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Terminal Complement Complex C5b-9 (C5b-9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.