Pig C5b-9(Terminal Complement Complex C5b-9) ELISA Kit

 

Pig C5b-9(Terminal Complement Complex C5b-9) ELISA Kit

Size

96T

Catalog no.

ELK7917

Price

738 EUR

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Assay length

3h

Standard

50ng/mL

Assay Type

Sandwich

Sensitivity

0.32ng/mL

Detection range

0.781-50ng/mL

Alternative Names

MAC; Membrane Attack Complex

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Research Area

Signal transduction;Metabolic pathway;Apoptosis;Infection immunity;

About

Pigs and the smaller guinea pigs are frequent used as models for humans.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Terminal Complement Complex C5b-9 (C5b-9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Terminal Complement Complex C5b-9 (C5b-9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Terminal Complement Complex C5b-9 (C5b-9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Terminal Complement Complex C5b-9 (C5b-9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.