General ADT(Androsterone) ELISA Kit

 

General ADT(Androsterone) ELISA Kit

Size

96T

Catalog no.

ELK7926

Price

526 EUR

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Assay length

2h

Sensitivity

144pg/mL

Standard

90000pg/mL

Detection range

352-90000pg/mL

Assay Type

Competitive Inhibition

Alternative Names

17-Ketosteroid; 17-KS; 3a-Hydroxy-17-Androstanone

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Androsterone (ADT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Androsterone (ADT) and unlabeled Androsterone (ADT) (Standards or samples) with the pre-coated antibody specific to Androsterone (ADT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Androsterone (ADT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Androsterone (ADT) in the sample.