Pig pPLA2(Phospholipase A2, Pancreas) ELISA Kit

3h

pancreas

Sandwich

2000pg/mL

13.5pg/mL

31.25-2000pg/mL

Enzyme & Kinase;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Pigs and the smaller guinea pigs are frequent used as models for humans.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

PLA2G1B; PLA2A; PPLA2; Phospholipase A2, Group IB; Group IB phospholipase A2; Phosphatidylcholine 2-acylhydrolase 1B

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Pancreas (pPLA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Pancreas (pPLA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Pancreas (pPLA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2, Pancreas (pPLA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.