3h
Pig GSTp(Glutathione S Transferase Pi) ELISA Kit
Pig GSTp(Glutathione S Transferase Pi) ELISA Kit
Assay length
Assay Type
Sandwich
Standard
4000pg/mL
Sensitivity
22.8pg/mL
Detection range
62.50-4000pg/mL
Alternative Names
GSTP1; GST-P1; DFN7; FAEES3; GST3
Research Area
Enzyme & Kinase;Metabolic pathway;Tumor immunity;Hepatology;
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About
Pigs and the smaller guinea pigs are frequent used as models for humans.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Pi (GSTp). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione S Transferase Pi (GSTp). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Pi (GSTp), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione S Transferase Pi (GSTp) in the samples is then determined by comparing the O.D. of the samples to the standard curve.