Dog HMGA1(High Mobility Group AT Hook Protein 1) ELISA Kit

3h

20ng/mL

Sandwich

0.115ng/mL

0.312-20ng/mL

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Signal transduction;Metabolic pathway;Tumor immunity;Developmental science;

HMG-R; HMGIY; High mobility group protein A1; High mobility group protein R; High mobility group protein HMG-I/HMG-Y

Canine or Canis Lupus is mostly Beagle used for drug research. ELK Biotech produces ELISA test kits and polyclonal antibodies.E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Mobility Group AT Hook Protein 1 (HMGA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Mobility Group AT Hook Protein 1 (HMGA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Mobility Group AT Hook Protein 1 (HMGA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Mobility Group AT Hook Protein 1 (HMGA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.