2h
General DHT(Dihydrotestosterone) ELISA Kit
General DHT(Dihydrotestosterone) ELISA Kit
Assay length
Standard
2500pg/mL
Sensitivity
13.3pg/mL
Detection range
30.9-2500pg/mL
Assay Type
Competitive Inhibition
Research Area
Endocrinology;Reproductive science;Hormone metabolism;
Alternative Names
5α-DHT; 5α-Dihydrotestosterone; Androstanolone; Stanolone
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Dihydrotestosterone (DHT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Dihydrotestosterone (DHT) and unlabeled Dihydrotestosterone (DHT) (Standards or samples) with the pre-coated antibody specific to Dihydrotestosterone (DHT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Dihydrotestosterone (DHT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Dihydrotestosterone (DHT) in the sample.