Cattle AOX1(Aldehyde Oxidase 1) ELISA Kit

 

Cattle AOX1(Aldehyde Oxidase 1) ELISA Kit

Size

96T

Catalog no.

ELK8012

Price

738 EUR

Buy at gentaur.com
Assay length

3h

Standard

20ng/mL

Assay Type

Sandwich

Sensitivity

0.107ng/mL

Detection range

0.312-20ng/mL

Research Area

Enzyme & Kinase;

Alternative Names

AO; AOH1; Azaheterocycle hydroxylase

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldehyde Oxidase 1 (AOX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aldehyde Oxidase 1 (AOX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aldehyde Oxidase 1 (AOX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aldehyde Oxidase 1 (AOX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.