3h
Pig TRF(Transferrin) ELISA Kit
Pig TRF(Transferrin) ELISA Kit
Assay length
Standard
20ng/mL
Assay Type
Sandwich
Sensitivity
0.147ng/mL
Detection range
0.312-20ng/mL
Research Area
Tumor immunity;Infection immunity;Immune molecule;
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Alternative Names
TF; Siderophilin; Serotransferrin; Beta-1 metal-binding globulin
About
Pigs and the smaller guinea pigs are frequent used as models for humans.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transferrin (TRF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transferrin (TRF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transferrin (TRF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transferrin (TRF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.