Rat ATP1b4(ATPase, Na+/K+ Transporting Beta 4 Polypeptide) ELISA Kit

 

Rat ATP1b4(ATPase, Na+/K+ Transporting Beta 4 Polypeptide) ELISA Kit

Size

96T

Catalog no.

ELK8016

Price

608 EUR

Buy at gentaur.com
Assay length

3h

Standard

10ng/mL

Assay Type

Sandwich

Sensitivity

0.055ng/mL

Detection range

0.156-10ng/mL

Research Area

Enzyme & Kinase;

Latin name

Rattus norvegicus

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Alternative Names

X,K-ATPase subunit beta-m; X/potassium-transporting ATPase subunit beta-m

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1β4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1β4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1β4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1β4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.