Rat ATP1b4(ATPase, Na+/K+ Transporting Beta 4 Polypeptide) ELISA Kit

3h

10ng/mL

Sandwich

0.055ng/mL

0.156-10ng/mL

Enzyme & Kinase;

Rattus norvegicus

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

X,K-ATPase subunit beta-m; X/potassium-transporting ATPase subunit beta-m

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1β4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1β4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1β4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, Na+/K+ Transporting Beta 4 Polypeptide (ATP1β4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.