3h
Pig MSTN(Myostatin) ELISA Kit
Pig MSTN(Myostatin) ELISA Kit
Assay length
Sensitivity
53pg/mL
Assay Type
Sandwich
Standard
8000pg/mL
Detection range
125.00-8000pg/mL
Alternative Names
GDF8; Growth Differentiation Factor 8
Research Area
Cytokine;Metabolic pathway;Bone metabolism;
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About
Pigs and the smaller guinea pigs are frequent used as models for humans.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myostatin (MSTN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myostatin (MSTN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myostatin (MSTN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myostatin (MSTN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.