General AED(Androstenediol) ELISA Kit

2h

53.4pg/mL

10000pg/mL

123.5-10000pg/mL

Competitive Inhibition

Endocrinology;Reproductive science;Hormone metabolism;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

5-AED; 5AED; 5-Androstenediol; Androst-5-Ene-3Beta,17Beta-Diol

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Androstenediol (AED) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Androstenediol (AED) and unlabeled Androstenediol (AED) (Standards or samples) with the pre-coated antibody specific to Androstenediol (AED). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Androstenediol (AED) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Androstenediol (AED) in the sample.